Table 1–
Effects of CNO and vehicle treatment in GFP-expressing rats
| Test | Reward Type | M-CNO | M-Veh | F-CNO | F-Veh |
|---|---|---|---|---|---|
| Mean (SEM) | Mean (SEM) | Mean (SEM) | Mean (SEM) | ||
| Homecage | 15% sucrose (ml/2hr) | 69.76 (4.94) | 66.58 (7.56) | 81.27 (16.75) | 81.07 (16.48) |
| 10% EtOH (g/kg/2hr) | 2.63 (0.38) | 2.42 (0.29) | 3.01 (0.25) | 2.55 (0.33) | |
| 20% EtOH (g/kg/2hr) | 1.54 (0.17) | 1.43 (0.11) | 2.10 (0.15) | 1.94 (0.22) | |
| FR1 Operant | 15% sucrose (trials initiated) | 127.50 (27.47) | 137.00 (27.15) | 124.00 (15.42) | 134.17 (10.05) |
| 10% EtOH (trials initiated) | 48.00 (9.25) | 33.17 (11.07) | 33.83 (6.15) | 49.00 (9.02) | |
| 20% EtOH (trials initiated) | 35.17 (6.12) | 28.33 (6.82) | 27.83 (2.83) | 31.00 (6.69) | |
| 15% sucrose (rewards collected) | 124.83 (17.45) | 133.33 (27.6) | 114.33 (27.58) | 128.83 (10.81) | |
| 10% EtOH (rewards collected) | 41.50 (11.70) | 28.33 (6.49) | 22.83 (9.3) | 34.17 (10.23) | |
| 20% EtOH (rewards collected) | 34.00 (5.93) | 27.00 (7.14) | 21.50 (3.25) | 23.00 (6.92) | |
| Cued-Reinstatement | 10% EtOH (total nosepokes) | 13.17 (2.77) | 19.00 (1.69) | 19.17 (4.84) | 16.83 (4.19) |
| 10% EtOH (trials initiated) | 10.83 (1.21) | 14.17 (2.77) | 16.17 (4.05) | 19.17 (2.77) | |
| 10% EtOH (latency to initiate trial) | 569.95 (132.41) | 540.77 (181.77) | 558.88 (131.68) | 559.85 (148.23) | |
| 10% EtOH (total well entries) | 154.00 (34.25) | 166.00 (44.23) | 169.83 (43.89) | 172.50 (38.61) | |
| 10% EtOH (well entries after cue) | 5.83 (2.56) | 8.33 (0.88) | 7.00 (1.16) | 10.67 (4.06) | |
| 10% EtOH (latency to enter well) | 1.41 (0.16) | 1.26 (0.17) | 1.37 (0.17) | 1.26 (0.15) | |
Mean and SEM of results of CNO and vehicle treatment for each behavioral measure in male and female rats expressing virally-transduced GFP as a control. The same data are presented here as are presented in graphical format for DREADD-expressing rats. All comparisons of CNO vs. vehicle treatment were non-significant except one: 10% EtOH (trials initiated). The details of these results are described in the text.