Figure 2:

B6N mice have a shorter poly(I:C) immune response. (A) Experiment schematic: To assess differences in poly(I:C) immune response timecourse between substrains, B6N and B6J mice were either injected with saline or poly(I:C) (5mg/kg) euthanized 3 h or 24 h post-injection, the mPFC freshly dissected, RNA extracted and qRT-PCR analysis was performed. (B) Heatmap showing transcript abundance for each group: saline, 3 h post-injection poly(I:C), 24 h post-injection poly(I:C). Levels for each transcript are shown as log2 fold-change values, normalized to Gusb (n=6/group). For the heatmap, yellow represents high transcript abundance, purple is low transcript abundance. Heirarchical clustering, one-way ANOVA, and Tukey’s post-hoc tests were used to analyze the data (**** P < 0.0001, *** P < 0.0002, ** P < 0.0021, * P < 0.05).