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(A) Elution profile of the cell lysate from a nickel affinity column (His-Trap HP column (5 ml). The bound protein was eluted with an increasing imidazole gradient over ~ 7 column volumes. (B) Elution profile from a Superdex 200 GL column of the combined pooled fractions from the previous His-Trap HP column step. Both profiles were monitored at 410 nm.
