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A variable temperature experiment was performed on recombinant human Cytc and Cytc-CTX. The thermal melting curves were measured by focusing on the 222 nm peak. (●) Cytc thermal denaturation data points, (▬) Cytc thermal denaturation fitting curve, (◊) Cytc-CTX thermal denaturation data points, (–) Cytc-CTX thermal denaturation fitting curve.
