Table 2.
Pseudo-first order rate constants for zinc catalyzed pNPA hydrolysis at pH 9.5 and Cu catalyzed NiR activity at pH 5.8.
| Construct | CuNiR (×10−4 s−1) | pNPA rate (×10−4 s−1) |
|---|---|---|
| TRIW-H | 4.7 | 22 |
| GRW-H | 28 | 52 |
| GRW27stamAELL33H | 112 | 3.5 |
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