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. Author manuscript; available in PMC: 2021 Oct 1.

Published in final edited form as: FASEB J. 2020 Aug 26;34(10):13935–13948. doi: 10.1096/fj.202001136R

Figure 6. — Caov-3, SKOV-3 and OVCAR-3 cells were treated for 24 hours with LPA as in Fig. 1 in the presence of vehicle, TNF-α Ab or TNF-α SR at the indicated concentrations. These TNF-α blocking agents were added simultaneously with LPA. Concentrations of IL-8 (A), IL-6 (B), CXCL1 (D) in culture supernatants were measured with ELISA and the results presented in the same way as TNF-α described in Fig. 1A. C. IL-6 protein in culture supernatants of Caov-3 cells or in 100X concentrated supernatants of SKOV-3 cells were assessed by immunoblotting analysis (upper). The time-dependent induction of intracellular IL-6 in the presence or absence of TNF-α SR was examined by immunoblotting analysis of cellular lysates (middle). The specificity of TNF-α SR inhibition of IL-6 was verified by lack of an inhibitory effect on cellular COX-2, another LPA-induced gene (lower).

Figure 6. — Caov-3, SKOV-3 and OVCAR-3 cells were treated for 24 hours with LPA as in Fig. 1 in the presence of vehicle, TNF-α Ab or TNF-α SR at the indicated concentrations. These TNF-α blocking agents were added simultaneously with LPA. Concentrations of IL-8 (A), IL-6 (B), CXCL1 (D) in culture supernatants were measured with ELISA and the results presented in the same way as TNF-α described in Fig. 1A. C. IL-6 protein in culture supernatants of Caov-3 cells or in 100X concentrated supernatants of SKOV-3 cells were assessed by immunoblotting analysis (upper). The time-dependent induction of intracellular IL-6 in the presence or absence of TNF-α SR was examined by immunoblotting analysis of cellular lysates (middle). The specificity of TNF-α SR inhibition of IL-6 was verified by lack of an inhibitory effect on cellular COX-2, another LPA-induced gene (lower).