Skip to main content
. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Dev Biol. 2020 Oct 13;469:46–53. doi: 10.1016/j.ydbio.2020.10.001

Fig. 2. Knockdown of nup205 Disrupts Cilia in Multiple Tissues.

Fig. 2.

(A) Representative images and quantitation of cilia number for control and nup205 morphant embryo LROs (ventral view with anterior to the top). Ns indicate number of distinct LROs analyzed. (B) Schematic of two-cell injections and representative images of control and nup205 morphant sides of an embryo (lateral view with dorsal to the top, top panel left side, bottom panel right side) and severity of ciliation defects in individual MCCs along with quantitation of remaining multiciliated structures and severity of MCC defect frequency. Ns indicate number of distinct embryos for which both sides were analyzed (dot plot) and numer of MCCs analyzed on either control MO or nup205 MO injected sides of embryos with no more than 10 MCCs assessed per a single side of an embryo (bar graph). Graph colors correspond to example border colors. Bar graph color coding depicts proportion of total MCCs assessed, while error bar is based on frequency of combined severe and abnormal phenotypes. (C) Representative localization of overexpressed GFP-NUP205 at the nuclear periphery and cilium in the Xenopus LRO and MCCs (yellow dotted line designates magnified area). p<0.0001 for **** by two-tailed T-Test for dot plots or Chi Squared Test for bar graph. Graphed values were obtained across three replicates.