Figure 8:

Rabbit anti-Qβ-glycan 1 sera enhance opsonophagocytosis of S. Paratyphi A. Log-phase cultures of S. Paratyphi A strain ATCC 9150 were first opsonized with heat-inactivated rabbit sera and then added to partially confluent monolayers of J774 macrophages. Gentamicin was then applied to kill non-phagocytosed bacteria, and internalized bacteria (represented as colony forming units [CFUs]) were enumerated by serial dilution and plating. Bars indicate the mean + standard deviation and are derived from four independent experiments. Statistical analysis was performed using a 2-tailed Student’s t-test. ***: p < 0.001; ****: p < 0.0001.