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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Alcohol Clin Exp Res. 2020 Aug 2;44(9):1734–1746. doi: 10.1111/acer.14415

Figure 1.

Figure 1.

(a) ‘Flow restriction’ model of arteriosclerosis. Placement of ligature on left common carotid, just distal to the bifurcation, is shown. (b) Lineage tracing experiments were performed using S100β-eGFP/Cre/ERT2–dTomato double transgenic mice (S100β-eGFP/tdTm), generated by crossing S100β-eGFP/Cre-ERT2 mice with Ai9 reporter mice. (c) Experimental protocol using S100β-eGFP/tdTm mice showing the time sequence of tamoxifen (Tx) administration (to induce Cre-LoxP recombination and indelibly mark resident S100β+ cells), EtOH or cornstarch (CS) gavage (initiated 1 week prior to ligation, and continued for two weeks post-ligation), and vessel harvest 14d post ligation. LL=left ligated, RCL=Right contralateral. (d) Over the course of the experimental protocol mouse body weights were not significantly different between control (CS) and EtOH groups (Mean +/− SD, n= 13).