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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Arthritis Rheumatol. 2020 Oct 11;72(11):1872–1881. doi: 10.1002/art.41400

Figure 2. Curli/eDNA complexes cross-react with lupus autoantigen dsDNA.

Figure 2.

ELISA for A) anti-dsDNA IgG Abs and (B) anti-curli/eDNA IgG Abs was performed on a representative SLE patient after co-incubation with calf thymus dsDNA or curli/eDNA complexes in suspension. C) ELISA used dsDNA as antigen and a mouse monoclonal Ab against dsDNA. D) ELISA used curli/eDNA as antigen and a rabbit polyclonal Ab against curli/eDNA complexes. In A-D, results are representative of 3 experiments. ELISA for anti-K12 E. coli DNA IgG (E), anti-calf thymus dsDNA IgG (F) and anti-curli/eDNA IgG Abs (G) in SLE patients anti-dsDNA Ab positive or negative by hospital records vs Healthy Controls (HC). Competitive ELISA with SLE plasma for anti-curli/eDNA IgG Abs (H) and anti-dsDNA Abs (I) after co-incubation with CsgA, curli/eDNA, and K12 DNA. Statistical significance was calculated with ANOVA and post-hoc t-test (E-I).