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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Mol Microbiol. 2020 Jun 29;114(4):563–581. doi: 10.1111/mmi.14560

Fig. 6. Compartment-specific ATP concentration is altered in channel mutants.

Fig. 6.

B. subtilis mutants unable to produce the indicated channel protein and engineered to synthesize Luc H245F in the MC or FS were starved to induce sporulation. At 2 h PS, culture aliquots were transferred to a 96-well plate, and at the indicated times PS the luminescence intensity was measured (Fig. S7) and the Luc H245F level was assessed by immunoblot analysis with anti-Luc antibodies (Fig. S8). For each biological replicate, the luminescence intensity was divided by the Luc H245F level to yield a normalized value representing the relative ATP concentration in the MC (A) or FS (B). Graphs show the average of three or four biological replicates and error bars represent one standard deviation. WT cell results are shown for comparison (same data as Fig. 3D). One asterisk (*) indicates P < 0.05 and two asterisks (**) indicate P < 0.005 in Student’s two-tailed t-tests comparing the data for each mutant with WT cells at the same time PS.