Table 1.
Morphology of bacterial strains after 6 h of starvation.
| Straina | Percentage of sporangia at each morphological stage | |||
|---|---|---|---|---|
| Flat septa | Curved septa | Early engulfment | Late or complete engulfment | |
| WT | 4 | 4 | 5 | 87 |
| spoIIQ | 13 | 7 | 59 | 21 |
| spoIIIAH | 9 | 12 | 42 | 37 |
| spoIIIAA | 8 | 5 | 4 | 83 |
a
B. subtilis wild-type (WT) or mutant strains engineered to produce Luc H245F in the MC or FS were starved to induce sporulation. At 2 h PS, culture aliquots were transferred to a 96-well plate containing FM 4-64 to stain membranes. Images were collected at 6 h PS using confocal fluorescence microscopy. The percentage of sporangia (150-250 counted; rod-shaped cells were not counted) at each morphological stage is listed for the strains synthesizing Luc H245F in the MC. Data for WT cells are from Figure 4. Gray fill indicates > 40%. Similar results were observed for the strains synthesizing Luc H245F in the FS.