TABLE 2.
Descriptive Data on Insulin Signaling and Related Measures
| Median (interquartile range) | |||||
|---|---|---|---|---|---|
| Variable | n | Total | Diabetes | Nondiabetes | Pairwise Difference Mean (95% CI) |
| ELISA | |||||
| pS307IRS1 | 150 | 0.10 (0.09, 0.10) | 0.10 (0.09, 0.10) | 0.10 (0.09, 0.10) | – |
| Total IRS1 | 150 | 0.28 (0.19, 0.40) | 0.26 (0.17, 0.40) | 0.33 (0.21, 0.44) | – |
| pT308AKT1 | 150 | 0.15 (0.13, 0.18) | 0.15 (0.13, 0.18) | 0.15 (0.13, 0.18) | – |
| Total AKT1 | 150 | 0.40 (0.20, 0.71) | 0.36 (0.20, 0.64) | 0.43 (0.23, 0.85) | – |
| pS307IRS1/total IRS1a | 150 | 0.34 (0.24, 0.49) | 0.39 (0.26, 0.53) | 0.31 (0.23, 0.46) | 0.05 (0.01, 0.09) |
| pT308AKT1/total AKT1a | 150 | 0.41 (0.23, 0.65) | 0.42 (0.24, 0.68) | 0.36 (0.20, 0.60) | 0.05 (−0.04, 0.14) |
| Immunohistochemistry | |||||
| IRS1 cells/mm2 | 139 | 5.72 (4.50, 7.29) | 5.90 (4.64, 7.98) | 5.30 (4.50, 6.91) | −0.8 (−2.63, 1.02) |
| Ex vivo stimulation | |||||
| IRS1 recruitment to IRβ | 78 | 4.66 (4.27, 5.10) | 4.69 (4.33, 5.12) | 4.55 (4.04, 5.10) | 0.19 (−0.13, 0.51) |
| pS473AKT1 | 78 | 4.45 (4.08, 4.81) | 4.67 (4.22, 4.93) | 4.35 (4.00, 4.69) | 0.24 (0.01, 0.46) |
| pT308AKT1 | 78 | 4.5 (4.09, 4.92) | 4.55 (4.16, 4.99) | 4.46 (3.87, 4.80) | 0.26 (0.04, 0.48) |
| pY1150/1151IRβ | 78 | 4.67 (4.21, 5.24) | 4.64 (4.27, 5.26) | 4.68 (4.11, 5.24) | 0.12 (−0.19, 0.44) |
| pY960IRβ | 78 | 4.53 (4.11, 4.97) | 4.64 (4.11, 5.33) | 4.42 (4.10, 4.88) | 0.18 (−0.11, 0.46) |
a
Among the ELISA measures, the ratios were the primary variables of interest.
CI = confidence interval; ELISA = enzyme-linked immunosorbent assay.