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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Inherit Metab Dis. 2020 Aug 5;43(6):1333–1348. doi: 10.1002/jimd.12290

FIGURE 5.

FIGURE 5

Yeast complementation assay using an ALG13 deficient yeast strain. A, An ALG13 deficient yeast strain was grown under selection conditions allowing for expression of wild type yALG13 when grown in the presence of galactose. Transfection and expression of various yALG13 mutants were performed as previously described.7 B, Western blot analysis of glycosylated carboxypeptidase Y under various complementation and growth conditions was performed in triplicates with representative data shown. C, Western blot analysis showing the transfected levels of yALG13-Flag tagged protein