FIGURE 5.

Inhibition of protein kinase C (PKC) or calcium calmodulin-dependent kinase II (CaMK) blocks maresin 1 (MaR1)-stimulated increase in [Ca²⁺]_i and high molecular weight glycoprotein secretion. Rat conjunctival goblet cells were incubated with the PKC inhibitor RO-317549 (10⁻⁷ M) or the CaMKII inhibitor KN93 (10⁻⁷ M) and its inactive control KN92 (10⁻⁷ M) for 30 min, then stimulated with MaR1 at 10⁻⁸ M for [Ca²⁺]_i or 10⁻⁹ M for secretion or carbachol (Cch, 10⁻⁴ M). The change in peak [Ca²⁺]_i (a and c) and secretion (b and d) are shown. Data are mean ± SEM from four (a), three (b), five (c), and three (d) experiments. *Significance above basal. #Significance from maresin 1 alone. [Ca²⁺]_i, intracellular Ca²⁺; SEM, standard error of the mean