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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Cell Physiol. 2020 Jun 8;236(1):340–353. doi: 10.1002/jcp.29846

FIGURE 6.

FIGURE 6

Effect of Inhibition of phospholipase D (PLD) and phospholipase A2 (PLA2) on maresin 1 (MaR1)-stimulated increase in [Ca2+]i and high molecular weight glycoprotein secretion. Rat conjunctival goblet cells were preincubated with the PLD inhibitor 0.3% 1-butanol or the inactive control 0.3% t-butanol for 15 min and stimulated with MaR1 at 10−8 M for [Ca2+]i or 10−9 M for secretion or carbachol (Cch, 10−4 M). Changes in peak [Ca2+]i are shown in (a) and secretion shown in (b). Goblet cells were incubated with the PLA2 inhibitor aristolochic acid (AA; 10−6–10−5 M) for 30 min and stimulated with MaR1 at 10−8 M for [Ca2+]i or 10−9 M for secretion or Cch (10−4 M). Data show changes in peak [Ca2+]i (c) while secretion is shown in (d). Data are mean ± SEM of four (a), three (b), eight (c), and three (d) experiments. *Significance from basal. #Significance from MaR1 alone. [Ca2+]i, intracellular Ca2+; SEM, standard error of the mean