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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Cell Physiol. 2020 Jun 8;236(1):340–353. doi: 10.1002/jcp.29846

FIGURE 8.

FIGURE 8

Inhibition of the cAMP-dependent protein kinase A (PKA) does not block maresin 1 (MaR1)-stimulated increase in [Ca2+]i, high molecular weight glycoprotein secretion, and MaR1 does not increase (cAMP). Goblet cells were preincubated with the cAMP-dependent PKA inhibitor H89 at 10−7–10−5 M concentrations for 30 min and stimulated with MaR1 at 10−8 M for [Ca2+]i and cAMP levels or 10−9 M for secretion or VIP (10−8 M). Changes in [Ca2+]i over time is shown in (a) and (b), while changes in peak [Ca2+]i are shown in (c). Secretion is shown in (d). The amount of cAMP in goblet cells is shown in (e). Data are mean ± SEM of four (a–c), seven (d), and five (e) experiments. *Significance above basal. #Significance from MaR1 or VIP alone. Arrow indicates addition of MaR1 or VIP. cAMP, cyclic adenosine monophosphate; [Ca2+]i, intracellular Ca2+; SEM, standard error of the mean