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. Author manuscript; available in PMC: 2020 Dec 8.
Published in final edited form as: Matrix Biol. 2020 Sep 17;94:77–94. doi: 10.1016/j.matbio.2020.09.001

Fig. 3.

Fig. 3.

Knockout of Col11a1 mRNA and protein expression in the Col11a1 tendon targeted conditional mice. (A) Quantitative real-time PCR shows comparable expression of Col11a1 mRNA in the wild type and Col11a1flox/flox controls. Col11a1 mRNA expression in Col11a1Δten/Δten FDLs is reduced to baseline while expression in the Col11a1+/Δten FDLs was ~50% of control values. Day 14 wild-type (n=3), Col11a1flox/flox (n=5), Col11a1+/Δten (n=8), Col11a1flox/flox (n=7) mice for each genotype. (B) Western blot analysis of Col11a1 protein (α1(XI)) content in control; wild type (n=3), Scx-Cre (n=4), Col11a1flox/flox (n=7) mice was done using Wes automated western blotting. The Col11a1+/Δten (n=3), and Col11a1Δten/Δten (n=4) FDL contained reduced and virtually no α1(XI) reactivity relative to controls. All 3 control mice contained comparable amounts of α1(XI). Day 4 mice. Left panel shows a representative image and the right panel presents the quantitation results from all mice.