Skip to main content
. Author manuscript; available in PMC: 2020 Dec 8.
Published in final edited form as: Matrix Biol. 2020 Sep 17;94:77–94. doi: 10.1016/j.matbio.2020.09.001

Fig. 9.

Fig. 9.

Induced knockdown Col11a1 in the mature tendon does not affect fibril structure. Knockdown of Col11a1 was induced using tamoxifen once daily for 3 days (i.p 10mg/100g body weight) beginning at day 25 and fibril structure analyzed at day 60. This approach allows the FDLs to develop as in wild type through development and into maturation. Knockdown is during maturation and mature FDLs are analyzed. (A,B) Electron microscopic analysis shows no significant effect on the fibril diameter and organization. Fibrils from both genotype have normal circular profiles. (C-E) The diameter distributions from wild type and I-Col11a1−/− FDLs are both bimodal and very similar. The larger diameter subpopulation is shifted modestly toward larger diameter in the I-Col11a1−/− compared to wild type FDLs. (F) Fibril density in I-Col11a1−/− FDLs is decreased, consistent with the increase in diameter observed. (n=3 mice for each genotype).