Fig. 3. Cerebrovascular cells are affected during sepsis-induced neuroinflammation.

a Fluorescence-activated cell sorting (FACS) strategy for the simultaneous sorting of gliovascular cells from an adult mouse brain, microglia, CECs, pericytes, astrocytes were defined and gated as CD45 low-mid CD11b+, CD45− CD13− CD31+, CD45− CD31− CD13+, and CD45− CD31− CD13− O4− ACSA2+ respectively, b–i Time course of mRNA fold change in microglia, astrocytes, CECs, and pericytes isolated from the brains of mice injected with 10 mg/kg LPS for 4 h and 24 h, b Tnf, c Il1a, d Il6, e Lcn2, f Ccl2, g Cxcl10, h Ptgs2, and i Socs3. Graphs depict mean ± SEM, n = 4 (females) each group, Two-way ANOVA with Dunnett post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001 asterisks indicate significance at that particular treatment time point as compared with the respective PBS group in each of the cell types.