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. 2020 Dec 7;8:219. doi: 10.1186/s40478-020-01092-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — H3.3G34V is associated with higher H3K27me3 enrichment. a Relative proportion of H3K27me3 enrichment across gene elements with H3.3G34V expression or knock-down in KNS42 cells. b Metagene profile of H3K27me3 enrichment at active enhancers (H3K27ac peaks 2.5 kb away from TSS). Higher H3K27me3 was observed in transduced KNS42 without DOX and non-transduced KNS42 compared to KNS42 with G34V knockdown. c At HOXA13, H3K27me3 enrichment is lower in KNS42 with G34V knockdown, compared to KNS42 no dox control and non-transduced KNS42. Blue tracks are cell lines that harbor, or overexpressed, H3.3G34V; red tracks are cell lines that do not harbor, or knockdown, H3.3G34V. d Metagene profile of H3K27me3 enrichment. H3K27me3 enrichment in the gene body across the top 1000 (top) most G34V-enriched genes in KNS42 TRIP no dox control. (bottom) most diff genes in G34V enrichment before and after KD in KNS42 TIRP ± DOX. e Heatmap profiles of K27me3 peaks at the top 1000 most K27me3 enriched loci. Signals from (left) KNS42 with DOX-induced G34V knockdown, (middle) KNS42 without G34V knockdown (no DOX control), and (left) the difference between them (Δ, left minus middle). Negative values in the third column indicate that KNS42 with DOX-induced G34V knockdown has lower K27me3 enrichment compared to no DOX control