Fig. 6.

Cerulenin inhibits LPS-induced microglia activation. Cerulenin (6 nmol per animal, solid bars) or vehicle (water) (open bars) was injected into the left ventricle. One hour later, LPS (3 μg per animal) or vehicle (CTL:1x PBS) was injected again. Brains were analyzed 3 h later. a Brains were perfusion-fixed and immunostained for iba-1. Images from cortex (CTX) and hippocampus (HIP) (top) were analyzed for iba-1 positive area and iba-1 positive cell number (bottom). Bar = 70 μm. b RNA was isolated from CTX and HIP and analyzed by RT-qPCR for TNF-α and IL-1β. Mean + SE, n = 3–4. #P < 0.05, compared to CTL. #P < 0.05, compared to CTL. *P < 0.05 compared to vehicle