Figure 8.

The C105F mutation leads to improved tubular repair 7 days following ischemia-reperfusion injury (IRI). Mice with indicated genotype underwent unilateral ischemia (28 minutes) and contralateral nephrectomy followed by reperfusion for 24 hours or 7 days. A: Western blot analysis of uromodulin (Umod) expression and corresponding densitometry in whole kidney lysates from indicated genotypes. B: Immunofluorescence of uromodulin expression in wild-type (WT) and mutant (mut) kidneys, as indicated. C: Enzyme-linked immunosorbent assay of plasma uromodulin levels at 24 hours following IRI in sham operated (control) and experimental mice. n = 10 per group (C). ∗P < 0.05, ∗∗∗P < 0.001 versus wt con. Scale bars = 50 μm (B). con, control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.