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. 2020 Dec;190(12):2436–2452. doi: 10.1016/j.ajpath.2020.08.015

Figure 9.

Figure 9

Mice with indicated genotype were examined 7 days following ischemia-reperfusion injury (IRI). A: Western blot analysis of uromodulin (Umod) expression and corresponding densitometry in whole kidney lysates from indicated genotypes. B: Enzyme-linked immunosorbent assay (ELISA) of plasma uromodulin levels at 7 days following IRI. C–F: Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining of wild-type (WT; C and D) and mutant (mut)/+ kidney (E) (D) with corresponding positive cell counts (F). Boxed area in C is shown at higher magnification in D. G–I: Kim 1 immunohistochemistry of kidney sections of WT (arrows indicate Kim-1 expressing tubules; G) and mut/+ mice (H) and corresponding urine Kim-1 ELISA (I) at day 7 following IRI (P value compared with sham operated WT mice). J: Western blot of NLRP3, phosphorylated p65 (p-p65), and caspase 11 expression in whole kidney lysates from indicated genotypes. K: IL-1β ELISA assay of whole kidney lysates at indicated time points following IRI (P value for WT IR 24 hours versus WT control (con), mut/+ IR 24 hours versus mut/+ con, and WT IR day 7 versus WT con). n = 10 per group (K). ∗P < 0.05 versus wt con; †P < 0.05, ††P < 0.01 versus wt IR D7; ‡‡‡P < 0.001 versus tg ir d7; §§P < 0.01 versus control. Scale bars: 100 μm (C and E); 50 μm (D); 500 μm (G and H). Cr, creatinine; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPF, low-power field.