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. 2020 Sep 24;133(21):2573–2585. doi: 10.1097/CM9.0000000000001101

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Copyright © 2020 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0

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(A) Alignment of the plasmid sequences of the K. pneumoniae strains. BLASTN-based whole genome comparison was performed and visualized using BRIG to exhibit the architecture and gene repertoire of a total of twelve K. pneumoniae plasmids, using the plasmid sequence of pF1_1 as a reference. Part of the IncFII plasmid backbone region, ProphagePlas1_1, ProphagePlas1_2, and the significant genes are indicated by rectangles. (B) Transposon carrying bla_KPC-2 located in our isolates (ΔΔTn1721-bla_KPC-2) was compared with that in pKPHS2 (ΔTn1721- bla_KPC-2) and that in the unnamed plasmid of SWU01 (ΔΔTn1721-bla_KPC-2). Regions of synteny between adjacent schematics are indicated by the shaded areas; the matching percentage nucleotide sequence identity for each such region is indicated. These schematics are drawn to scale. K. pneumoniae: Klebsiella pneumoniae.