Fig. 2. Analysis of immune cell involvement in regulating ID8/Ubr5^−/− tumor growth.

a–c Peritoneal washes were collected at indicated times from OC bearing mice after peritoneal implantation of ID8 cells (n = 5 mice per group). CD4⁺ (a) /CD8⁺ (b) T cells were analyzed on CD45⁺ infiltrating cells. c Foxp3⁺CD25⁺ regulatory T cells were analyzed on CD45⁺CD3⁺CD4⁺ tumor infiltrating T cells (TILs). d B and T cell deficiency did not mitigate the differences in growth capacity between control and Ubr5^−/− tumors. Proportion of Muc16⁺ CD45⁻ tumor cells in ascites were quantified at day 30 post OC implantation (n = 5 mice per group). e, f Representative FACS images of infiltrated CD11b⁺F4/80⁺ macrophages in peritoneal cavity or lung at day 30 after tumor injection. Proportion of macrophages on CD45⁺ infiltrating cells in peritoneal cavity (e) (n = 5 mice per group), and lung (f) (n = 3 mice per group) were quantified. g Representative images of CD68⁺ macrophages and surrounded Ki67⁺ cells. All panels are the same magnification, scale bars: 50 μm. h–i CD68⁺ cells (h) and Ki67⁺ cells (i) in individual and spheroid populations were quantified (n = 5 mice per group and ten confocal images acquired from each sample). j At day 30 post i.p. injection, spheroids from peritoneal washes were harvested and evaluated by H&E staining (n = 5 mice per group and an average of 10 fields acquired from each sample). Scale bars: 200 μm. In all cases, data are representative of at least two independent experiments with similar results. Data are presented as mean ± SEM, unpaired two-sided Student’s t-test with no correction for multiple comparison, *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.