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. 2020 Dec 8;10:21397. doi: 10.1038/s41598-020-78167-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nerve fibers near regenerated HCLCs. Samples collected at 4 weeks after adenovirus (Ad) and DT-induced HC (HC) ablation. Spiral ganglion neuron (SGN) fibers were visualized by Neurofilament 200 (NF200) staining. Myosin VIIa (MyoVIIa) and tdTomato (tdTom) double-positive cells were associated with nerve fibers along the cell body. Both groups—adenovirus with Atoh1 (Ad.Atoh1) and with Atoh1 + Gfi1 (Ad.Gfi.Atoh1) showed similar association with SGNs. Representative images are shown from 4 biological replicates for each group. Scale bar = 100 μm.