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. 2020 Dec 8;6:140. doi: 10.1038/s41420-020-00378-9

Fig. 5. CD63 participated in FN-EDA induced angiogenesis.

Fig. 5

A Immunoblot result of HUVECs treated with FN-EDA and/or irigenin for 6 h. (Data are shown as mean ± SEM; pFAK: FN-EDA versus Irigenin+FN-EDA: **P < 0.001, FN-EDA versus Ctrl: **P < 0.001; pSrc: FN-EDA versus Irigenin+FN-EDA: **P < 0.001, FN-EDA versus Ctrl: **P < 0.001.) B Immunoblot result of HUVECs treated with FN-EDA and/or Src inhibitor SU6656 for 6 h. (Data are shown as mean ± SEM; pVEGFR2: FN-EDA + SU6656 versus FN-EDA: *P < 0.01; pSrc: FN-EDA versus Ctrl: ns; pSrc: FN-EDA + SU6656 versus FN-EDA: **P < 0.001; pSrc: FN-EDA versus Ctrl: ns.) C Immunoblot result of HUVECs lysis immunoprecipitated by anti-flag antibody. HUVEC were treated with rEDA (40 μg/ml) for 6 h before harvest. D Tube formation assay of HUVECs transfected with CD63-siRNA alone or both CD63-siRNA and CD63-plasmid before treated with FN-EDA (40 μg/ml) for 6 h (n = 3). (Data are shown as mean ± SEM; CD63-siRNA versus CD63-siRNA+ CD63-plasmid: *P < 0.01 CD63-siRNA versus Ctrl-siRNA: *P < 0.01; scale bar = 100 μm.) E Migration assay of HUVECs transfected with CD63-siRNA alone or both CD63-siRNA and CD63-plasmid before treated with FN-EDA (40 μg/ml) for 24 h (n = 3). (Data are shown as mean ± SEM; CD63-siRNA versus CD63-siRNA+ CD63-plasmid: **P < 0.001 CD63-siRNA versus Ctrl-siRNA: **P < 0.001. Scale bar = 100 μm.) F Immunoblot result of HUVECs transfected with CD63-siRNA alone or both CD63-siRNA and CD63-plasmid before treated with FN-EDA (40 μg/ml) for 6 h. (Data are shown as mean ± SEM; pVEGFR2: CD63-siRNA versus CD63-siRNA+CD63-plasmid: *P < 0.01; CD63-siRNA versus Ctrl: **P < 0.001; pFAK: CD63-siRNA versus CD63-siRNA+CD63-plasmid: ns; CD63-siRNA versus Ctrl: ns; pSrc: CD63-siRNA versus CD63-siRNA+CD63-plasmid: ns; CD63-siRNA versus Ctrl: ns; CD63: CD63-siRNA versus CD63-siRNA+CD63-plasmid: ***p < 0.0001; CD63-siRNA versus Ctrl: ***p < 0.0001.) G Immunohistochemical staining of CD63 in mice fibrosis hepatic tissues. Immunofluorescence shows CD63 (red) and CD31 (green) were co-stained.