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. 2020 Dec 8;10:21486. doi: 10.1038/s41598-020-78473-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Doxorubicin exposure modulates tight junctional gene expression in a time dependent manner. RT-qPCR of T84 cell mRNA at 9 h and 24 h after DXR exposure with time respective controls. The experimental design is the same as Fig. 1A. All data is presented as fold change normalized to ACTB. n = 6 wells/ group. Statistics: 2 way ANOVA followed by Tukey’s HSD; ***p < 0.001.