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. 2020 Dec 8;10:21475. doi: 10.1038/s41598-020-78359-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IEC cultured under microfluidic conditions show higher expression of genes involved in digestion, intestinal transport and hormone metabolic process. (A) Heatmap representation of the top 30 differentially expressed genes between standard and microfluidic HIO culture in three HIO donors. Higher levels of expression are represented in red and lower levels in blue. (B) Differentially-expressed genes between standard and microfluidic HIO culture were grouped under Gene ontology (GO) functional pathways. Circle size represents the number of genes differentially expressed in each GO pathway and lines link individual genes to their related GO pathways.