Table 2.
Composition of the HISC medium.
| Constituent | Supplier (Catalog number) | Final concentration |
|---|---|---|
| rh R-spondin 3 | R&D systems (3500-RS/CF) | 0.5 µg/mL |
| rh Noggin | PeproTech (120-10C) | 0.1 µg/mL |
| B27 supplement | Gibco (17504) | 1X |
| N-Acetyl-L-cysteine | Sigma Aldrich (A9165) | 1.25 mM |
| Nicotinamide | Sigma Aldrich (N0636) | 0.01 M |
| rh EGF | PeproTech (AF-100-15) | 0.05 µg/mL |
| rh Gastrin 1 | Tocris (3006) | 0.005 µM |
| Primocin | InvivoGen (ant-pm) | 0.1 mg/mL |
| A83-01 | Tocris (2939) | 0.5 µM |
| SB202190 | Sigma Aldrich (S7067) | 0.01 mM |
| Y-27632 | Abmole (M1817) | 0.01 mM |
| Wnt3a conditioned medium | - | 50% |