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Changes in HELLS localization follow disruption of the H3K9me3 pathway. Representative confocal laser scanning microscopy images of live wild-type and Suv39H1/H2−/− MEFs post-transient transfection with either wild-type HELLS (a) or HELLS K237Q (b). (c) Quantification of localization patterns observed in wild-type or mutant HELLS transfected cells as representatively shown in (a) and (b). We analyzed 50–60 cells per cell type for every construct.
