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. 2020 Nov 12;21(12):1145–1153. doi: 10.1080/15384047.2020.1840886

Figure 1.

Figure 1.

CYP2C8 was anti-proliferative and indicative of favorable survival in liver cancer. (a) The expression of CYP2C8 in LIHC tumor dataset (red) and normal dataset (gray) was plotted. (b) Kaplan-Meier survival analysis illustrated the relationship between CYP2C8 expression and overall survival from LIHC dataset. (c and d) LC cells from four cell lines and normal cells underwent qRT-PCR analysis to detect CYP2C8 mRNA (c) and protein (d) levels. (e) HepG2 and Huh-7 cells transfected with pcDNA3.1 or pcDNA3.1/CYP2C8 underwent qRT-PCR analysis to detect CYP2C8 mRNA level. (f) The proliferation of HepG2 and Huh-7 cells transfected with pcDNA3.1 or pcDNA3.1/CYP2C8 was tested by EdU assay. (g) CCK-8 assay monitored the reduced viability of HepG2 and Huh-7 cells after CYP2C8 overexpression. Optical density (OD) at 450 nm was regarded as the measurement of cell viability. (h) TUNEL assay evaluated the enhanced apoptosis of HepG2 and Huh-7 cells after the transfection of CYP2C8 overexpression plasmids. (i) Flow cytometry analysis examined the influence of CYP2C8 overexpression on LC cell apoptosis. p < .05 (*), p < .01 (**), p < .001 (***)