Figure 2.

CYP2C8 was directly targeted by miR-382-3p. (a) According to the prediction of miRDB, top three miRNAs (miR-4797-5p, miR-382-3p and miR-3665) with higher target score with CYP2C8 were selected. The alteration of CYP2C8 mRNA levels in HepG2 and Huh-7 cells were evaluated in response to the transfection of mimics or inhibitors of these miRNAs. (b) CYP2C8 protein levels in LC cells were measured in response to miR-382-3p overexpression or inhibition by western blot. (c) RIP assay revealed that CYP2C8 mRNA and miR-382-3p could be enriched with Ago2 protein. (d) The sequences of wild-type CYP2C8 3ʹ-UTR, mutated CYP2C8 3ʹ-UTR and miR-382-3p were shown. The binding sites were highlighted in red. (e) Luciferase reporter assay showed that miR-382-3p overexpression inhibited the luciferase activity of CYP2C8-WT reporters in HepG2 and Huh-7 cells. (f) RNA pull-down demonstrated that CYP2C8 mRNA could be enriched by bio-miR-382-3p probes. p < .01 (**), p < .001 (***)