Figure 4.

CYP2C8 elevation counteracted the impact of GAS5 on LC cell proliferation and apoptosis. (a) The knockdown efficiency of CYP2C8 and the overexpression efficiency of GAS5 in HepG2 cells were measured by qRT-PCR and western blot. (b) CCK-8 assay indicated that CYP2C8 knockdown reversed the inhibitory effect of GAS5 overexpression on HepG2 cell viability. (c) EdU assay showed that CYP2C8 silencing facilitated HepG2 cell proliferation which had been suppressed by GAS5 overexpression. (d) TUNEL assay presented that CYP2C8 knockdown abolished the enhanced HepG2 cell apoptosis altered by GAS5. (e) Flow cytometry analysis evaluated the cell apoptotic level of the transfected cells. p < .01 (**), N.S.: not significant