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. 2020 Dec 8;10:21491. doi: 10.1038/s41598-020-77990-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A2 and A3-mediated inhibition is alphavirus-specific and cell-type-independent. (A) and (B) Infectious titers of the VEEV TrD strain in HMC3 and U87MG cells respectively, reported as PFU/mL. Cells were pre-treated with peptides (pA2/pA3) for 2 h prior to infection; or cells were treated with peptides and immediately co-infected with virus. (C) EEEV (GA97 strain) titers in U87MG cells following treatment with peptides A2 and A3. (D). RVFV titers (MP-12 strain) in Vero cells following treatment with peptides A2 and A3. All treatments were performed at a concentration of 10 µg/mL of each peptide and infected with respective virus at MOI = 0.1. n = 3; *p > 0.05; **p > 0.01; ***p > 0.001; ****p > 0.0001.