Figure 3.

Tissue-specific MT-A and GFP expressions. Neonatal Daphnia (< 24 h) were exposed to different concentrations of Zn²⁺ for 24 h. Midgut and hepatopancreas of D. magna MetalloG were dissected and mRNA was purified. mRNA was also purified from the remaining tissue. The mRNA expression levels of MT-A and GFP were estimated by qPCR. Relative amounts of mRNA to that of the ribosomal L32 protein gene were estimated by ΔΔCτ method and indicated in Y-axis. The quantification was performed three times. Bar = SE.