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. 2020 Dec 8;10:21490. doi: 10.1038/s41598-020-78572-z

Figure 5.

Figure 5

Dose depenent GFP expression changes after heavy metal exposure for 1 h or 24 h. Five daphniids were exposed in 10 mL of ADaM containing indicated metal ions for 1 h or 24 h. GFP expression was quantified and normalized to the control (0 µg/L) expression level. Numbers on x-axis indicate concentrations of each heavy metal (µM for Zn2+ and nM for Cu2+ and Cd2+). Y-axis indicates fold changes. Arrows indicate ECIR1.5 values. Top: 1 h exposure, Bottom: 24 h exposure. N = 5; ***p < 0.001; **p < 0.01; *p < 0.05 (t-test).