Fig. 5. Mitochondrial function remains unaltered in Acly^M-KO BMDMs.

a Differentiated control and AclyM-KO macrophages (BMDMs) were left untreated or stimulated with LPS (b) relative abundance of TCA cycle intermediates ***P  <  0.0001. c Lactate secretion after 24 h LPS treatment. d Metabolized glucose in 24 h. e Cellular energy charge as calculated by [ATP] + .05[ADP]/([AMP] + [ADP] + [ATP]). f Cellular ATP concentration. g Seahorse analyses and extracted parameters. Values represent mean ± SEM from n  = 12 technical replicates from two combined experiments b, n =  3 technical replicates from three pooled mice from one of three representative experiments (c, d, g), n = 6 technical replicates from three pooled mice from one of two representative experiments (e, f). ***P < 0.001 by two-way ANOVA with Bonferroni post hoc test for multiple comparisons. Source data are deposited in  MTBLS2159 (b) or provided as a Source Data file (c–g).