Fig. 7. Deletion of Acly leads to deregulation of lipid mediators and decreased responsiveness to prostaglandin E2.
a Total sum of measured lipids by metabololipidomics. b Neutral lipids analyzed by Bodipy 493/503. c Sum of AA, DHA, EPA, AdA, LA, ALA/GLA, DPAn-3, and DGLA omega 3 and 6 fatty acids (FA’s) measured by metabololipidomics. d Lipid abundances and genes involved in prostaglandin synthesis. e Ptger4 expression in control and Acly-deficient macrophages. f Effect of PGE2 treatment on cytokine secretion in control and AclyM-KO LPS-treated BMDMs. g Effect of PGE2 treatment on cytokine secretion in control and AclyM-KO LPS-treated BMDMs. Values represent mean ± SEM (n = 3 technical replicates from three pooled mice a–e), or mean (n = 2 technical replicates from three pooled mice (f, g)) Source data are provided as a Source Data file (a–d, f) or deposited in GSE126690 (d, e).