Skip to main content
. 2020 Dec 8;11:6290. doi: 10.1038/s41467-020-20067-6

Fig. 2. Characterization of Coupa in living cells via SIM imaging.

Fig. 2

a, c Mitochondria co-stained with Coupa (405 nm excitation) and MTG (488 nm excitation) in untreated (a) and CCCP-treated HeLa cells (c). b, d Zoom-in images of white rectangles in a and c; e CCCP-treated HeLa cells co-stained with Coupa (561 nm excitation) and LTG (488 nm excitation); f Zoom-in images of white rectangle in e. g Schematic representation of Coupa staining mitochondria (Coupa-mito) and lysosomes (Coupa-lyso) simultaneously in living cells; photobleaching of Coupa-mito and MTG (h) and Coupa-lyso and LTG (i) upon continuous irradiation by 561-nm and 488-nm lasers. White dotted lines indicate region of interest for fluorescence measurement. The time-dependent fluorescence intensity was shown in the lower right panel. MTG channel: Ex, 488 nm, Em, 500–550 nm; LTG channel: Ex, 488 nm, Em, 500–550 nm; Coupa-lyso channel: Ex, 561 nm, Em, 570–640 nm, and Coupa-mito channel: Ex, 405 nm, Em, 420–495 nm.