Fig. 2. Molecular cloning of TGW1.

a Venn diagram to show distributions of homozygous SNPs in three groups of homozygous BC₁F₂ mutants. S1 to S3 represented three groups of homozygous BC₁F₂ mutants. Each number represents numbers of homozygous SNPs. Only two nonsynonymous homozygous SNPs presented in three groups. b The expression of KAT-2B (TraesCS6B01G432600) and KAT-2A (TraesCS6A01G392400) in different tissues at various developmental stages. n = 3 independent replicates. c Genomic structure of TGW1 (KAT-2B) gene. The red triangle points to the position of the point mutation in tgw1. d Verification of the SNP in the KAT-2B (TGW1) gene in WT and tgw1 by sequencing. e Contents of JA in leaves of WT and tgw1. n = 3. f Relative expression level of Cell Number Regulator 6 (CNR6) in WT and tgw1 mutant. n = 4. g The expression level of CNR6 upon 10 μM JA treatments in WT. n = 4. h The expression level of CNR6 upon 200 μM JA inhibitor DIEC treatments in WT. n = 4. Data are represented as mean ± SEM in b, and e–h. p values are indicated by two-tailed unpaired t test in e–h. Source data underlying Figs. 2a, b, e–h are provided as a Source Data file.