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. 2020 Dec 8;10:21404. doi: 10.1038/s41598-020-78417-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AtSUC2-CcFT3 transgenic rootstock flowering phenotype. (A) Precocious flowering from a 16-month old transgenic AtSUC2-CcFT3 plant. (B) Closeup of fruit set following self-pollination of the flowers. (C) A view of the whole plant. (D) Precocious flowering in a self-pollinated AtSUC2-CcFT3 F1 seedling within a year of seed germination. (E) Precocious in vitro flowering within a month of transformation using a 35S-CcFT3 construct and (F) relative CcFT3 gene expression of transgenic lines transformed with the different constructs. The error bars represent the means of different transgenic lines. Different letters represent a significant difference at P < 0.05 using Student's t-Test.