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. 2020 Dec 8;10:21404. doi: 10.1038/s41598-020-78417-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular analysis of Citrus transgenic lines expressing CcFT3 under control of the AtSUC2 promoter. (A) Southern blot of selected CcFT3 lines indicating the transgene copy number. The lanes were loaded as following: (1) WT or non-transgenic control, (2) AtSUC2-CcFT3 MP4, (3) AtSUC2-CcFT3 MP3, (4) AtSUC2-CcFT3 MP2 and (5) AtSUC2-CcFT3 MP1. (B) CcFT3 transcript quantification from the selected citrus transgenic lines. CsACTIN was used as reference gene. Data represent the mean (± standard deviation, SD) of three technical replicates and different letters represent a significant difference at P < 0.05 using Student's t-Test (C–F) Western blot showing CcFT3 protein quantification (C) in the mother plants (MP) and (D–F) two individual self-pollinated seedling progenies (SDL) from each MP line. The membranes were probed with CcFT3 specific antibody and Ponceau-S was used as loading control.