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. 2020 Dec 8;10:21404. doi: 10.1038/s41598-020-78417-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of flowering pathway related genes. (A–E) Relative expression of flowering related genes for each of the 16-month old transgenic lines compared to the non-transgenic Carrizo citrange shown by RT-qPCR (A) CONSTANS (CO), (B) SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), (C) TERMINAL FLOWER1 (TFL1), (D) APETALA1 (AP1) and (E) LEAFY (LFY). RNA was extracted from leaf petioles rich in phloem tissues. (F–H) Total RNA was extracted from leaf tissues to show the expression flowering pathway genes by RT-qPCR (A) SOC1, (B) AP1 and (C) LFY in the AtSUC2-CcFT3 transgenic lines compared to the Carrizo citrange non-transgenic line. CsACTIN was used as the reference gene. Data represent the mean (± standard deviation, SD) of three technical replicates. Different letters represent a significant difference at P < 0.05 using Student's t-Test.