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. 2020 Dec 8;10:21404. doi: 10.1038/s41598-020-78417-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Precocious flowering one-year old non-transgenic ‘Valencia’ scion grafted onto AtSUC2-CcFT3 transgenic rootstock (A) Flower bud emergence within 21 days following budding. Insert shows enlarged image of emerging flower buds (B) fully expanded flower buds, (C) fully open flowers, (D) developing sweet orange fruit and (E) relative FT gene expression in the scion leaves at different stages of flowering. Data represent the mean (± standard deviation, SD) of three technical replicates. Different letters represent a significant difference at P < 0.05 using Student's t-Test.