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. 2020 Dec 8;10:21404. doi: 10.1038/s41598-020-78417-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A volcano plot of DEGs and validation of the transcriptome analysis of selected DEGs using RT-qPCR. (A) Volcano plot showing the Log₂ fold change difference and the adjusted P-value for 21,635 genes included on all platforms. The red dots represent upregulated genes and the blue dots the downregulated genes in AtSUC2-CcFT3 MP3 transgenic lines (adjusted P < 0.05). (B) The expression levels of DEG candidates in AtSUC2-CcFT3 MP3 transgenic line was calculated using 2^-ΔΔCt and those values were compared to non-transgenic control values. Different letters (a, b) represent a significant difference at P ≤ 0.05 using Duncan’s Multiple Range Test and error bars represent SE (n = 3) for RT-qPCR (black) and RNAseq (blue).