Figure 5.

Similar activation of wild-type and Ldlr deficient platelets after transplantation of Ldlr-deficient mice. Bone marrow transplantations were performed using bone marrow from either wild-type (WT^BM) or Ldlr^−/− mice (Ldlr^−/−BM) injected into irradiated Ldlr^−/− recipients, held on normal chow diet. (A) Isolated platelets were activated by thrombin (0.5–4 nM) or cross-linked collagen-related peptide (CRP-XL, 0.5–5 μg/ml), and exposure of PS, P-selectin and activation of integrin α_IIbβ₃ were determined by flow cytometry using AF647-labeled annexin A5, FITC-labeled anti-CD62P mAb, or PE-labeled JON/A mAb, respectively. Means ± SEM (n = 4–8 animals/group). (B) PPACK-anticoagulated blood was perfused over collagen type I at wall shear rate of 1000 s⁻¹. Brightfield images were captured, after which the deposited platelets were stained for integrin α_IIbβ₃ activation, P-selectin expression and PS exposure in different colors. Platelet activation parameters were obtained, as described for Fig. 1. Left: Scaled heatmap with integration of age groups. Right: Scaled heatmap of effect sizes per genotype. Effect size per parameter was calculated from the pooled standard deviation, Cohen’s d and regression coefficient r.