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. 2020 Dec 8;11:6287. doi: 10.1038/s41467-020-20058-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Experimental design used to generate a third genetic model, Δ3w mice. The indicated groups of mice were administered tamoxifen (Tam.) at P13 and tissue was collected four weeks later. b Single myofiber images (left) and quantified average number of nuclei per myofiber (right) in Δ3w and control EDL muscle at P42. Control myofibers have an average of 246 nuclei/myofiber, while Δ3w mice have an average of 180 nuclei/myofiber. Myonuclei are labeled with DAPI. c Representative sections of Δ3w and control tibialis anterior (TA) muscle at P42 (left). Analysis of cross-sectional area (right) showed Δ3w myofibers are 22% smaller than controls. Sections in (c) were immunostained with laminin antibodies (red) and stained with DAPI (white). d Average length of Δ3w EDL myofibers is decreased by 9% compared to controls. e Myofiber volume showed a 33% reduction in Δ3w EDL at P42. 20 myofibers were analyzed per animal (n = 3 biologically independent animals) in (b), (d), and (e). At least 3 20× images were analyzed per mouse in (c) (n = 6 biologically independent animals). Statistical analyses and data presentation: (b–e) two-sided unpaired t-test; *p < 0.05, **p < 0.01. Data are represented as mean ± SD. Scale bars: (b) 200 μm, (c) 50 μm. Source data are provided as a Source Data file.