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. Author manuscript; available in PMC: 2020 Dec 8.

Published in final edited form as: Biochemistry. 2015 Mar 16;54(11):2001–2010. doi: 10.1021/acs.biochem.5b00044

Table 2.

Binding of p53(35–59) Mutant Peptides to Taz2

	K_a (M⁻¹)	ΔH (kcal/mol)	ΔS (cal mol⁻¹ deg⁻¹)
p53 WT	(1.14 ± 0.13) × 10⁶	−2.70 ± 0.02	18.9 ± 0.3
p53 M40A	(1.32 ± 0.05) × 10⁶	−2.35 ± 0.06	20.4 ± 0.3
p53 L45A	(0.91 ± 0.04) × 10⁶	−3.05 ± 0.28	17.4 ± 1.0
p53 D48N	(0.60 ± 0.08) × 10⁶	−3.13 ± 0.10	16.3 ± 0.1
p53 I50A	(0.31 ± 0.01) × 10⁶	−2.66 ± 0.18	16.5 ± 0.6
p53 E51Q	(0.56 ± 0.13) × 10⁶	−2.19 ± 0.52	19.2 ± 1.3
p53 Q52E	(1.29 ± 0.35) × 10⁶	−2.07 ± 0.03	21.2 ± 0.5
p53 W53Q	ND^a	ND^a	ND^a
p53 F54S	(0.53 ± 0.09) × 10⁶	−1.59 ± 0.63	21.0 ± 1.7
p53 T55A	(1.16 ± 0.10) × 10⁶	−2.57 ± 0.03	19.4 ± 0.1
p53 pThr55	(1.30 ± 0.05) × 10⁶	−1.9 ± 0.46	21.8 ± 1.6

^a

No binding was detected.